Cleaver COMET Assay Tanks

  • For Single Cell Gel Electrophoresis.
  • Minimise exposure to light and reduce background DNA damage.
  • High efficiency cooling for enhanced resolution.


Cleaver Scientific Comet Assay Tanks are available in four slide formats to study single cell gel electrophoresis (SCGE), a technique made popular by drug toxicology and carcinogenesis studies for the detection and quantification of DNA damage in cells. Each tank’s robust construction from ebony acrylic ensures that cells remain free of exposure to background light and DNA damage during electrophoresis, while a cooled central platform provides a convenient surface for slide preparation and control of slide temperature during the assay. Following electrophoresis DNA damage may be measured using Comet Assay scoring software.

Background: First introduced in 1981 to quantify double-stranded DNA breakages in single cells exposed to irradiation, the Comet Assay (or SCGE) has since been adapted to analyse specific DNA lesions and repair processes.

Overview: Following genotoxic insult, such as ionizing radiation, the resultant strand breakage of supercoiled duplex DNA reduces the size of the large genomic DNA from which these strands are separated or drawn out by electrophoresis. The genomic DNA then takes on the appearance of a comet as its negatively charged broken ends and fragments migrate towards the anode during electrophoresis.

Method: After exposure to a genotoxic insult cells are suspended within low melting point agarose and embedded within a thin layer of agarose on a microscope slide. Cellular protein is then removed by lysis in detergent, when DNA is allowed to unwind in alkaline conditions before electrophoresis. The DNA is electrophoresed, stained and then analysed using software.

Results: Microscopy imaging is used to measure DNA fluorescence upon staining. In DNA damaged cells the resultant image resembles a ‘comet’ with the cellular DNA separated into a head and tail. The head is mainly composed of intact genomic DNA, whereas any fragmented or damaged DNA is concentrated within and towards the tail.


Cat. No.




Unit Dimensions (cm)

17 x 34 x 9

31 x 34 x 9

33 x 59 x 9

Slide Capacity




Base Buffer Volume




Typical Running Conditions

25V/300mA for 1h

25V/300mA for 1h

25V/300mA for 1h



Image Product Name Catalog No. Product Description Pack Size Quantity Action
Cleaver COMET Assay Tanks CSL-COM10

Comet Assay Tank for 10 Slides

Cleaver COMET Assay Tanks CSL-COM20

Comet Assay Tank for 20 Slides

Cleaver COMET Assay Tanks CSL-COM40

Comet Assay Tank for 40 Slides


Cat. No. Description Qty/Pack
STAINDISH Ebony treatment dish, pk/1 Each
STAINDISH4X Ebony treatment dish, pk/4 Each
CSL-CHILLPLATE Chill Plate For Comet Assay Slides Each
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