Cleaver RunView (RealTime) Electrophoresis

  • Real time visualisation of DNA migration using blue light
  • Power supply integrated within the base unit
  • Multichannel compatible 20- / 28-sample combs for rapid screening
  • Can be purchased as an upgrade for multiSUB Choice

Overview

Using RunVIEW is simple; first, cast a gel featuring two rows of wells with one matching pair of the preparatory combs supplied, before transferring the blue-light transparent gel tray to the MSCHOICE tank located on the base unit. Add sufficient buffer just to cover the gel, and remove the combs to load the DNA samples into the upper row of wells (loading tier). Replace the lid to connect the MSCHOICE tank to the integrated power supply before applying the voltage and switching on the blue LED gel illuminator. Watch through the bluVIEW lid viewing pane as the samples migrate in real-time to the second row of wells (extraction tier). Once the DNA bands of interest enter the tier, simply stop the power supply, remove the lid and harvest the DNA by pipette.

Track DNA without harmful UV


Blue light is safe and has none of the detrimental effects of UV transillumination, including mutagenesis which can compromise cloning efficiency. RunVIEW’s capacity to provide real-time visualisation of electrophoresis enables DNA to be tracked as it migrates through the gel. This allows the user to judge precisely when the band of interest is ready for extraction.

Save on time-consuming gel elution techniques


Running DNA into a well containing buffer effectively purifies the DNA of agarose, eliminating the need for time-consuming gel excision and purification techniques that also result in sample loss. Once harvested, the DNA needs only to be cleaned by a straightforward ethanol precipitation when it is ready for further digestion or ligation.

No expensive commercial gels


RunVIEW works with standard EtBr, SYBR Green and SYBR Safe gels cast within the 15×7, 15×10 or 15x15cm MSCHOICE gel trays, and therefore does not require expensive precast gels and accessories.

A self-contained system


The base unit, which houses the in-built power supply and blue LED gel illuminator, is compact, dual-voltage and portable, and allows electrophoresis, gel visualisation and extraction to be performed at the bench, without the inconvenience of having to transport gels to a darkroom elsewhere within the laboratory.

Specifications

Cat. No. CSL-RVMSCHOICE10 CSL-RVMSCHOICE15 CSL-RVMSCHOICE7 CSL-RVMSCHOICETRIO
Unit Dimensions (cm) 29.3 x 22 x 8 29.3 x 22 x 9 29.3 x 22 x 10 29.3 x 22 x 11
Timer 1-999 minutes with alarm 1-999 minutes with alarm 1-999 minutes with alarm 1-999 minutes with alarm
Transilluminator Wavelength 470 470 470 470
Package Includes MSCHOICE10 MSCHOICE15 MSCHOICE7 MSCHOCETRIO
Input Voltage 100-240V, 50/60Hz 100-240V, 50/60Hz 100-240V, 50/60Hz 100-240V, 50/60Hz
Operating Temperature Ambient to 40°C Ambient to 40°C Ambient to 40°C Ambient to 40°C
Safety Features No load detection No load detection No load detection No load detection
Power 30 30 30 30
Maximum Voltage (V) 150 150 150 150
Maximum Current (mA) 300 300 300 300
Operating Modes Constant Voltage or Current Constant Voltage or Current Constant Voltage or Current Constant Voltage or Current
Maximum Sample Capacity 140 210 70 210
Base Buffer Volume 500 500 500 500
Typical Running Conditions 90-150V, 60-90 minutes 90-150V, 60-90 minutes 90-150V, 60-90 minutes 90-150V, 60-90 minutes
Gel Dimensions 15 x 10 15 x 15 15 x 7 15 x 7 & 15 x 10 & 15 x 15

Video

Variations

Image Product Name Catalog No. Product Description Pack Size Quantity Action
Cleaver RunView (RealTime) Horizontal Electrophoresis CSL-RVMSCHOICE7

runVIEW® CHOICE complete with 15 x 7cm gel tray and 1x 4/16MC and 1x 20/28MC 3mm preparatory, 2x 4/16MC sample and 4x 20/28MC 1mm thick, Inbuilt Power Supply and Blue LED Transilluminator

Cleaver RunView (RealTime) Horizontal Electrophoresis CSL-RVMSCHOICE10

runVIEW® CHOICE complete with 15 x 10cm gel tray and 1x 4/16MC and 1x 20/28MC 3mm preparatory, 2x 4/16MC sample and 4x 20/28MC 1mm thick, Inbuilt Power Supply and Blue LED Transilluminator

Cleaver RunView (RealTime) Horizontal Electrophoresis CSL-RVMSCHOICE15

runVIEW® CHOICE complete with 15 x 15cm gel tray and 1x 4/16MC and 1x 20/28MC 3mm preparatory, 2x 4/16MC sample and 4x 20/28MC 1mm thick, Inbuilt Power Supply and Blue LED Transilluminator

Cleaver RunView (RealTime) Horizontal Electrophoresis CSL-RVMSCHOICETRIO

runVIEW® CHOICE complete with 15x7cm, 15x10 & 15x15 gel tray and 1x 4/16MC and 1x 20/28MC 3mm preparatory, 2x 4/16MC sample and 4x 20/28MC 1mm thick, Inbuilt Power Supply and Blue LED Transilluminator

Accessories

Cat. No. Description Qty/Pack
CSL-RVBSBVLID runVIEW base station & bluVIEW lid. Each
CSL-RVGELDOC runVIEW® Gel Documentation Hood with 24.1 MP camera Each
CSL-RVLID runVIEW bluVIEW lid - For Safe Dyes & EtBr Each
CSL-RVHOOD runVIEW VIEWING HOOD Each
CSL-RVGELDOCSYS runVIEW gel Documentation hood with camera, Laptop & 1D Analysis Software Each
CSL-RVSTATION runSTATION complete with CSL-RVGELDOC & CSL-RVMSCHOICETRIO Each
CSL-RVGDCOMPLETE RVGDCOMPLETE includes RVGELDOCSYSTEM and RVMSCHOICETRIO Each
* For Trays, Combs and Electrophoresis Accessories please refer to Cleaver Wide Midi Horizontal Electrophoresis Section (MSCHOICE)
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