Overview
The Mini omniPAGE Sub Blot systems are complete stand-alone units for submarine blotting. Each unit comprises an omniPAGE tank and lid, electroblotting insert, and cassettes and fibre pads. The versatile design of the omniPAGE tank and lid allows the unit to be easily adapted for vertical PAGE or IEF with capillary tube gels, using the relevant optional insert. Other features include:
- Multi-transfer capability for 4 Mini gels in the Mini system
- Complete run-time flexibility from overnight to as short as a couple of hours
- Cool pack for environmentally friendly low-cost cooling during high-intensity transfers
- Open design, rigid cassettes maximise current transfer and eliminate shadow band formation
- Cassettes colour-coded prevent polarity reversal to ensure transfer in the correct orientation
The Maxi format omniPAGE Sub Blot system is a complete stand-alone unit for submarine blotting. Each unit comprises an omniPAGE tank and lid, electroblotting insert, and cassettes and fibre pads. The versatile design of the omniPAGE tank and lid allows the unit to be easily adapted for vertical PAGE or IEF with capillary tube gels, using the relevant optional insert. Other features include:
- Multi-transfer capability for up to 4 Maxi gels, 8 Mini Wide and 16 Mini gels
- Complete run-time flexibility from overnight to as short as a couple of hours
- Cool pack for environmentally friendly low-cost cooling during high-intensity transfers
- Open design, rigid cassettes maximise current transfer and eliminate shadow band formation
- Cassettes colour-coded prevent polarity reversal to ensure transfer in the correct orientation.
High Intensity Blotters
The Sub Blot Mini and WAVE high intensity transfer systems combine the cooling capacity of wet submarine blotting units with the speed of semi-dry transfer systems. Both systems utilise plate electrodes to create a higher strength electric field and greater current density than conventional wire-electrode systems. This allows transfer of broad range molecular weight proteins to be achieved typically within an hour in the Sub Blot Mini, and in 1-2 hours using the WAVE Sub Blot System. Features offered by the Sub Blot Mini and WAVE are as follows:
- Plate-electrodes 2cm apart in the Sub Blot Mini facilitate fast transfer of two stacked 8×8.5cm mini gels; and 4cm apart in WAVE Sub Blot system for transfer of one Maxi gel
- Multi-transfer capability in the WAVE also allows transfer of 4 omniPAGE Mini and 2 omniPAGE Mini Wide gels arranged side-by-side
- Two cooling options; a cool pack supplied with the Sub Blot Mini for environmentally friendly low-cost cooling; and active cooling in the Sub Blot WAVE by a detachable cooling coil connected to a recirculating chiller.
- Magnetic stirring bars fit conveniently beneath the cooling coil within the WAVE tank to maximise buffer circulation and heat dissipation, while preventing ion gradient formation; both tanks sit comfortably on most stirring plates
- Open design, rigid cassettes maximise gel-to-membrane compression for efficient current transfer; colour-coded to prevent polarity reversal
ElectroBlot Modules (for 5 Blots)
Designed primarily for wet electroblotting of proteins, electroBLOT Electroblotters offer a combination of increased capacity with economy saving features. Both units, Mini 10 x 10cm and Maxi 20 x 20cm, have increased capacity over standard systems with up to five gel blot cassettes utilised at any one time. This is especially useful in high throughput laboratories.
A uniform electric field is provided by a high intensity coiled electrode and ensures uniform transfer across the blot surface. The cassette open architecture ensures the maximum blot area allows direct transfer of current. Its rigid construction ensures contact between the gel and membrane is retained throughout the blot and an even pressure is maintained. These units are compatible with magnetic stirrers to aid heat dispersal and prevent pH drifts in the buffer due to incomplete buffer mixing. Each system includes a cooling pack to further enhance transfer efficiency by removing excess heat. This also saves on buffer for added economy.
