NEST Disposable Vacuum Filtration System is useful for sterilizing the large volume sample for tissue culture media and many other biological buffers which cannot be autoclavable. The unit includes PVDF membrane filters with graduated filter tops made with polystyrene, and Acrylonitrile-Butadiene-Styrene necks with polystyrene receiving bottles. A separated sterile polyethylene cap is included. Filtration system is manufactured from raw material of highly transparent polystyrene (PS). Filtration system comes in three different volumes: 250mL / 500mL / 1000mL.